Editorial 
and 
Author 
Guidelines 
for 
Publication 
in 
PROTEOMICS 


These 
guidelines 
outline 
issues 
that 
authors 
must 
follow 
when 
submitting 
a 
paper 
for 
publication 
in 
PROTEOMICS. 
Failure 
to 
follow 
these 
guidelines 
may 
be 
grounds 
for 
an 
Editorial 
decision 
to 
reject 
a 
manuscript 
without 
review. 
Authors 
are 
also 
requested 
to 
take 
note 
of 
the 
different 
types 
of 
manuscripts 
that 
are 
suitable 
for 
PROTEOMICS 
as 
detailed 
in 
the 
‘Instructions 
to 
Authors’. 


Experimental 
design 
and 
data 
analysis 
for 
2-D 
PAGE 
and 
MS-based 
experiments 


– 
The 
experimental 
design 
must 
be 
provided 
and 
must 
include 
details 
of 
the 
number 
of 
biological 
and 
analytical 
replicates. 
Only 
one 
biological/analytical 
replicate 
will 
not 
be 
acceptable. 
In 
clinical 
studies, 
it 
is 
highly 
desirable 
that 
a 
power 
analysis 
predicting 
the 
appropriate 
sample 
size 
for 
subsequent 
statistical 
analysis 
of 
the 
data 
is 
carried 
out. 
– 
For 
expression 
analysis 
studies, 
summary 
statistics 
(mean, 
standard 
deviation) 
must 
be 
provided 
and 
results 
of 
statistical 
analysis 
must 
be 
shown. 
Reporting 
fold 
differences 
alone 
is 
not 
acceptable. 
Authors 
must 
report 
the 
following: 
methods 
of 
data 
normalization, 
transformation, 
missing 
value 
handling, 
the 
statistical 
tests 
used, 
the 
degrees 
of 
freedom 
and 
the 
statistical 
package 
or 
program 
used. 
Where 
biologically 
important 
differences 
in 
protein 
(gene) 
expression 
are 
reported, 
confirmatory 
data 
(e.g. 
from 
immunoassays) 
are 
desirable. 
– 
For 
biomarker 
discovery/validation 
studies, 
the 
sensitivity 
and 
specificity 
of 
the 
biomarker(s) 
should 
be 
provided 
wherever 
possible. 
It 
is 
desirable 
that 
receiver 
operator 
characteristic 
(ROC) 
curves 
and 
areas 
under 
the 
curves 
are 
given. 
Protein 
identification 
and 
characterization 


– 
The 
method(s) 
used 
to 
generate 
the 
mass 
spectrometry 
data 
must 
be 
described, 
as 
should 
the 
methods 
used 
to 
create 
peak 
lists 
from 
raw 
MS 
or 
MS/MS 
data. 
– 
The 
name 
and 
version 
of 
the 
program 
used 
for 
database 
searching, 
the 
values 
of 
critical 
search 
parameters 
(e.g. 
parent 
ion 
and 
fragment 
mass 
tolerance, 
cleavage 
rules 
used, 
allowance 
for 
number 
of 
missed 
cleavages) 
and 
the 
name 
and 
version 
of 
the 
database(s) 
searched 
must 
be 
provided. 
INSTRUCTIONS 
TO 
AUTHORS 


Revised 
December 
2007 


Authors 
are 
requested 
to 
follow 
these 
Instructions 
and 
the 
Editorial 
and 
Author 
Guidelines 
carefully. 
Manuscripts 
not 
prepared 
accordingly 
will 
not 
be 
accepted. 


1 
Aims 
and 
scope 
2 
General 
terms 
of 
publication 
3 
Online 
submission 
of 
manuscripts 
4 
Types 
of 
contributions 
5 
Organization 
of 
manuscripts 
6 
Proofs 
and 
reprints 
7 
Funding 
information 
8 
Standard 
abbreviations 


1 
Aims 
and 
scope 


PROTEOMICS 
is 
the 
premier 
international 
source 
for 
information 
on 
all 
aspects 
of 
applications 
and 
technologies 
in 
proteomics. 
Its 
mission 
is 
to 
integrate 
the 
various 
areas 
of 
this 
rapidly 
developing 
field 
which 
is 
having 
a 
major 
impact 
on 
all 
areas 
of 
the 
life 
sciences 
and 
industry. 
We 
welcome 
papers 
describing 
novel 
applications 
of 
proteomics. 
Topics 
include 
whole 
proteome 
analysis 
of 
any 
organism, 
expression 
profiling, 
disease 
studies, 
pharmaceutical, 
agricultural 
and 
biotechnological 
applications, 
and 
analysis 
of 
cellular 
systems, 
organelles 
and 
protein 


ª 
2008 
WILEY-VCH 
Verlag 
GmbH 
& 
Co. 
KGaA, 
Weinheim 


– 
For 
each 
protein 
identified, 
measures 
of 
certainty 
(e.g. 
p-values) 
must 
be 
provided. 
For 
MS/MS, 
the 
number 
of 
peptides 
used 
to 
identify 
a 
protein 
must 
be 
given 
as 
well 
as 
the 
sequence 
and 
charge 
state 
of 
each 
peptide. 
For 
peptide 
mass 
fingerprinting, 
the 
number 
of 
peptides 
that 
match 
the 
sequence 
and 
the 
total 
percent 
of 
sequence 
coverage 
must 
be 
quoted. 
If 
extensive, 
the 
above 
information 
should 
be 
collected 
as 
supporting 
information 
which 
is 
available 
online. 
– 
For 
experiments 
with 
large 
MS/MS 
data 
sets, 
estimates 
of 
the 
false 
positive 
rates 
are 
required 
(e.g. 
through 
searching 
randomized 
or 
reversed 
sequence 
databases). 
This 
information 
should 
be 
provided 
as 
supporting 
information. 
– 
Where 
post-translational 
modifications 
are 
reported, 
the 
methods 
used 
to 
discover 
the 
modification 
must 
be 
described. 
The 
modification 
should 
be 
mapped 
to 
amino 
acid(s) 
by 
fragmentation 
analysis, 
but 
reported 
as 
ambiguous 
if 
mapping 
to 
a 
single 
amino 
acid 
is 
not 
possible. 
For 
isobaric 
modifications, 
evidence 
for 
assigning 
a 
specific 
modification 
must 
be 
provided 
and 
the 
spectra 
included 
as 
supporting 
information. 
– 
Where 
protein 
sequence 
isoforms 
are 
reported, 
the 
peptide 
sequence 
that 
matches 
the 
unique 
amino 
acid 
sequence 
of 
a 
particular 
isoform 
must 
be 
provided. 
Fragmentation 
analysis 
of 
the 
appropriate 
peptides 
should 
be 
described. 
Bioinformatics 


– 
Where 
a 
manuscript 
describes 
an 
academic 
database 
or 
software, 
it 
must 
be 
either 
freely 
accessible 
via 
the 
Internet, 
or 
downloadable 
and 
the 
access 
options 
must 
be 
provided. 
This 
also 
applies 
to 
commercial 
software 
or 
databases. 
Supporting 
information 


– 
Supporting 
information 
is 
encouraged. 
This 
includes 
protein 
identification 
results, 
expression 
data, 
and 
mass 
spectrometry 
peak 
lists. 
Note 
that 
all 
data 
must 
be 
in 
processed, 
not 
raw, 
form. 
This 
material 
will 
not 
be 
published 
in 
the 
journal 
but 
will 
be 
available 
online 
at 
the 
PROTEOMICS 
website 
(www.proteomics-journal.com). 
complexes. 
Technological 
topics 
covered 
include 
new 
or 
improved 
separation 
techniques, 
methods 
of 
protein 
identification 
and 
characterization 
including 
the 
analysis 
of 
post-translational 
modifications, 
transcriptomics 
and 
bioinformatics. 
PROTEOMICS 
publishes 
articles 
in 
English. 
Manuscripts 
must 
be 
grammatically 
and 
linguistically 
correct, 
and 
authors 
less 
familiar 
with 
English 
usage 
are 
advised 
to 
seek 
the 
help 
of 
English-speaking 
colleagues. 
Either 
American 
or 
English 
spelling 
is 
acceptable. 
PROTEOMICS 
is 
published 
in 
24 
issues 
per 
year, 
including 
regular 
issues 
as 
well 
as 
topical 
issues. 


2 
General 
terms 
of 
publication 


The 
author 
vouches 
that 
the 
work 
has 
not 
been 
published 
elsewhere, 
either 
completely, 
in 
part, 
or 
in 
any 
other 
form 
and 
that 
the 
manuscript 
has 
not 
been 
submitted 
to 
another 
journal. 
The 
submitting 
author 
(listed 
under 
“Correspondence”) 
accepts 
the 
responsibility 
of 
including 
as 
coauthors 
all 
appropriate 
persons. 
The 
submitting 
author 
certifies 
that 
all 
coauthors 
have 
seen 
a 
draft 
copy 
of 
the 
manuscript 
and 
agree 
with 
its 
publication. 


Upon 
acceptance 
of 
the 
manuscript 
the 
author 
is 
required 
to 
fill 
in 
the 
“Copyright 
Transfer 
Agreement” 
and 
the 
“Color 
and 
Page 
charge 
Agreement” 
forms 
(please 
see 
the 
journal 
homepage 
for 
current 
charges), 
sign 
them 
and 
submit 
them 
along 
with 
hardcopies 
of 
the 
illustrations 
of 
the 
paper 
to: 


www.proteomics-journal.com 



PROTEOMICS 
Editorial 
Office 
Wiley-VCH 
Verlag 
Boschstrasse 
12 
D-69469 
Weinheim 
Germany 
E-mail: 
proteomics@wiley-vch.de 
Fax: 
149-6201-606-172 


These 
mandatory 
forms 
can 
be 
found 
directly 
on 
the 
homepage 
of 
the 
journal 
at 
http://www.proteomics-journal.com 
under 
the 
link 
“For 
Authors”. 
Please 
note 
that 
if 
you 
are 
submitting 
material 
which 
has 
already 
been 
published 
elsewhere, 
you 
must 
also 
send 
to 
the 
Editorial 
Office 
permission 
in 
writing 
that 
this 
material 
may 
be 
reprinted 
in 
PROTEOMICS. 
Authors 
are 
expected 
to 
carry 
any 
costs 
arising 
from 
permissions. 


All 
scientific 
contributions 
will 
be 
peer-reviewed 
on 
the 
criteria 
of 
originality 
and 
quality. 
Authors 
may 
suggest 
up 
to 
five 
potential 
referees 
(please 
provide 
their 
e-mail 
addresses) 
as 
well 
as 
individuals 
whom 
they 
wish 
to 
be 
excluded 
from 
the 
review 
process. 
On 
acceptance, 
papers 
may 
be 
subjected 
to 
editorial 
changes. 
A 
revised 
paper 
will 
retain 
its 
original 
date 
of 
receipt 
only 
if 
it 
is 
resubmitted 
to 
the 
Editors 
within 
two 
months 
after 
revision 
was 
requested. 
Responsibility 
for 
the 
factual 
accuracy 
of 
a 
paper 
rests 
entirely 
with 
the 
author. 


Our 
Early 
View 
online 
publication 
is 
updated 
weekly 
and 
enables 
papers 
to 
be 
available 
online 
and 
citable 
before 
going 
into 
print. 


All 
instances 
of 
publishing 
misconduct, 
including, 
but 
not 
limited 
to, 
plagiarism, 
data 
fabrication, 
image/data 
manipulation 
to 
falsify/ 
enhance 
results 
etc. 
will 
result 
in 
rejection/retraction 
of 
the 
manuscript. 


3 
Online 
submission 
of 
manuscripts 


PROTEOMICS 
offers 
a 
web-based 
manuscript 
submission 
and 
peer-
review 
system. 
This 
service 
guarantees 
fast 
and 
safe 
submission 
of 
manuscripts 
and 
rapid 
assessment. 
Using 
this 
system 
is 
obligatory; 
conventional 
submission 
of 
manuscripts 
is 
no 
longer 
accepted. 


3.1 
General 
remarks 
To 
submit 
your 
manuscript 
online, 
please 
proceed 
as 
follows: 


– 
Prepare 
your 
manuscript 
and 
illustrations 
in 
the 
appropriate 
format, 
according 
to 
the 
instructions 
given 
below 
(see 
Sections 
4 
to 
8). 
Please 
also 
make 
sure 
that 
your 
paper 
conforms 
with 
the 
scientific 
and 
style 
instructions 
of 
PROTEOMICS 
as 
given 
herein. 
You 
can 
also 
find 
a 
link 
to 
these 
instructions 
on 
the 
homepage 
of 
the 
journal 
at 
http://www.proteomics-journal.com 
under 
the 
link 
“For 
Authors”. 
Links 
for 
English 
language 
assistance 
are 
also 
provided 
here. 
– 
If 
you 
have 
not 
already 
done 
so, 
create 
an 
account 
for 
yourself 
in 
the 
system 
at 
the 
submission 
site 
http://mc.manuscriptcentral.com/ 
proteomics/ 
by 
clicking 
on 
the 
“Create 
Account” 
button. 
– 
Let 
the 
system 
guide 
you 
through 
the 
submission 
process. 
Online 
help 
is 
available 
at 
all 
times 
during 
the 
process. 
You 
are 
also 
able 
to 
exit/re-enter 
at 
any 
stage 
before 
finally 
“submitting” 
your 
work. 
All 
submissions 
are 
strictly 
confidential. 
To 
monitor 
the 
progress 
of 
your 
manuscript 
throughout 
the 
review 
process, 
just 
login 
periodically 
and 
check 
your 
Author 
Center. 
If 
you 
have 
any 
questions 
concerning 
the 
online 
submission 
program, 
do 
not 
hesitate 
to 
contact 
Editorial 
Support 
at 
proteomics@wileyvch.
de. 


3.2 
Electronic 
manuscripts 
Please 
follow 
the 
instructions 
in 
Section 
5 
“Organization 
of 
manuscripts” 
when 
preparing 
the 
electronic 
version 
of 
the 
manuscript 
and 
ensure 
that 
data 
are 
given 
in 
the 
correct 
order 
and 
style 
for 
the 
journal. 


ª 
2008 
WILEY-VCH 
Verlag 
GmbH 
& 
Co. 
KGaA, 
Weinheim 


– 
Main 
text 
(incl. 
front 
material) 
as 
well 
as 
figure 
legends 
and 
tables 
(in 
this 
order) 
should 
be 
given 
in 
one 
file, 
preferably 
saved 
in 
.doc 
or 
.rtf 
format 
(Word 
2003 
or 
older, 
not 
.docx). 
– 
Data 
should 
be 
typed 
unjustified, 
without 
hyphenation 
except 
for 
compound 
words. 
Use 
carriage 
returns 
only 
to 
end 
headings 
and 
paragraphs; 
spacing 
will 
be 
introduced 
by 
the 
typesetter. 
– 
Do 
not 
use 
the 
space 
bar 
to 
make 
indents; 
where 
these 
are 
required 
use 
the 
TAB 
key. 
– 
If 
working 
in 
Word 
for 
Windows, 
please 
create 
special 
characters 
through 
Insert/Symbol. 
– 
Figures 
should 
preferably 
be 
in 
TIFF, 
EPS, 
PPT 
or 
the 
original 
format. 
Each 
figure 
should 
be 
given 
in 
a 
separate 
file 
and 
should 
have 
the 
following 
resolution: 
Type 
Resolution 


Graphs 
800 
– 
1200 
DPI 
Photos 
400 
– 
800 
DPI 
Color 
(only 
CMYK) 
300 
– 
400 
DPI 


All 
submissions 
will 
be 
converted 
to 
PDF 
format 
during 
the 
upload 
process. 
The 
system 
automatically 
generates 
one 
PDF 
file 
which 
contains 
all 
parts 
of 
the 
manuscript. 
It 
is 
not 
necessary 
to 
submit 
the 
graphics 
with 
high 
resolution 
at 
this 
stage. 


3.3 
Revised 
manuscripts 
In 
revised 
manuscripts 
the 
areas 
containing 
the 
major 
required 
changes 
should 
be 
marked 
and 
the 
script 
color 
changed. 
The 
file(s) 
with 
the 
changes 
visible 
on 
screen 
should 
be 
submitted 
to 
the 
online 
procedure. 


Upon 
acceptance 
of 
the 
manuscript 
the 
final 
uploaded 
version 
will 
be 
taken 
as 
the 
basis 
for 
copy 
editing 
and 
the 
subsequent 
production 
process. 


4 
Types 
of 
contributions 


Seven 
types 
of 
scientific 
contributions 
are 
considered 
for 
publication: 


(i) 
Research 
articles 
describing 
complete 
investigations. 
Unsolicited 
research 
articles 
should 
not 
exceed 
6500 
words 
in 
total; 
this 
includes 
references, 
figure 
legends 
and 
tables. 
Papers 
of 
up 
to 
7 
printed 
pages 
will 
be 
published 
free 
of 
charge; 
for 
papers 
exceeding 
that 
length 
a 
page 
charge 
(see 
the 
journal 
homepage) 
will 
be 
levied. 
Please 
note 
that 
the 
length 
of 
an 
article 
depends 
greatly 
on 
the 
type 
of 
figures 
and 
tables 
provided. 
Manuscripts 
must 
not 
have 
been 
published 
previously, 
except 
in 
the 
form 
of 
a 
preliminary 
communication. 
(ii) 
Review 
articles 
are 
normally 
invited 
by 
the 
Editor-in-Chief. 
Authors 
wishing 
to 
submit 
a 
review 
article 
should 
send 
a 
brief 
outline 
of 
its 
contents 
to 
Prof. 
Dunn 
(eic.proteomics1@ucd.ie) 
before 
the 
manuscript 
is 
drafted. 
(iii) 
Rapid 
communications 
describing 
results 
that 
are 
brief, 
timely 
and/or 
of 
such 
importance 
that 
rapid 
publication 
is 
warranted. 
These 
manuscripts 
should 
bear 
the 
words 
“Rapid 
Communication” 
immediately 
above 
the 
title 
on 
the 
first 
page. 
They 
should 
not 
be 
subdivided 
into 
titled 
sections 
but 
should 
be 
written 
in 
a 
continuous 
style. 
Rapid 
communications 
should 
normally 
not 
exceed 
four 
printed 
pages 
and 
contain 
no 
more 
than 
two 
figures 
and 
one 
table. 
(iv) 
Technical 
briefs 
describe 
the 
development 
of 
a 
novel 
method 
or 
an 
improvement 
or 
noteworthy 
modification 
of 
an 
already 
existing 
technique 
or 
platform 
used 
in 
proteomic 
analysis. 
These 
manuscripts 
should 
bear 
the 
words 
“Technical 
Brief” 
immediately 
above 
the 
title 
on 
the 
first 
page. 
A 
technical 
brief 
is 
a 
short 
(no 
more 
than 
two 
pages 
when 
published) 
description 
written 
in 
a 
continuous 
style 
with 
no 
more 
than 
two 
figures 
and 
one 
table. 
www.proteomics-journal.com 



(v) 
Dataset 
briefs 
describe 
novel 
proteomic 
datasets 
of 
specific 
types 
of 
samples, 
such 
as 
organisms, 
tissues, 
organelles, 
and 
cells. 
These 
datasets 
can 
be 
generated 
with 
any 
proteomic 
platform 
including 
two-dimensional 
gels, 
mass 
spectrometry 
or 
protein 
arrays. 
An 
important 
criterion 
is 
that 
the 
dataset 
contains 
a 
significant 
number 
of 
identified 
proteins 
that 
will 
benefit 
further 
research 
on 
that 
particular 
sample 
type. 
The 
manuscripts 
should 
bear 
the 
words 
“Dataset 
Brief” 
immediately 
above 
the 
title 
on 
the 
first 
page. 
They 
should 
not 
be 
subdivided 
into 
titled 
sections 
but 
should 
be 
written 
in 
a 
continuous 
style. 
Dataset 
briefs 
should 
normally 
not 
exceed 
two 
printed 
pages 
and 
contain 
no 
more 
than 
two 
figures 
and 
one 
table 
in 
the 
published 
text, 
but 
authors 
are 
encouraged 
to 
submit 
supporting 
information, 
such 
as 
annotated 
two-
dimensional 
gel 
images 
and 
tables 
of 
protein 
identifications, 
that 
will 
only 
appear 
online. 
(vi) 
Viewpoint 
articles 
are 
intended 
to 
stimulate 
discussion 
and 
debate 
in 
areas 
of 
general 
concern 
and 
controversy 
in 
proteomics, 
and 
generally 
reflect 
the 
personal 
opinions 
of 
the 
author(s). 
These 
manuscripts 
should 
bear 
the 
word 
“Viewpoint” 
immediately 
above 
the 
title 
on 
the 
first 
page. 
They 
should 
not 
be 
subdivided 
into 
titled 
sections 
but 
should 
be 
written 
in 
a 
continuous 
style. 
Viewpoint 
articles 
should 
normally 
not 
exceed 
two 
printed 
pages 
and 
contain 
no 
more 
than 
one 
figure. 
Potential 
authors 
considering 
contributing 
a 
viewpoint 
article 
to 
the 
journal 
should 
in 
the 
first 
instance 
contact 
the 
Editor-in-Chief 
by 
e-mail 
(eic.proteomics1@ucd.ie) 
to 
discuss 
their 
proposal. 
In 
order 
to 
provide 
a 
forum 
for 
debate 
of 
the 
issues 
raised 
in 
the 
viewpoint 
articles, 
correspondence 
concerning 
these 
articles 
will 
be 
published 
for 
a 
special 
area 
on 
the 
journal’s 
homepage 
(http://viewpoint.proteomics-journal.com). 
(vii) 
Reports 
on 
meetings, 
workshops, 
and 
other 
events 
of 
relevance 
to 
the 
field 
of 
proteomics 
are 
invited. 
These 
manuscripts 
should 
bear 
the 
word 
“Report” 
immediately 
above 
the 
title 
on 
the 
first 
page. 
They 
should 
not 
be 
subdivided 
into 
titled 
sections 
but 
should 
be 
written 
in 
a 
continuous 
style. 
Report 
articles 
should 
normally 
not 
exceed 
two 
printed 
pages 
and 
may 
contain 
a 
single 
figure 
to 
illustrate 
the 
meeting. 
Potential 
authors 
considering 
contributing 
a 
report 
article 
to 
the 
journal 
should 
in 
the 
first 
instance 
contact 
the 
Editor-in-Chief 
by 
e-mail 
(eic.proteomics1@ucd.ie) 
to 
discuss 
their 
proposal. 
5 
Organization 
of 
manuscripts 


Manuscripts 
must 
be 
typewritten 
with 
double 
spacing 
(including 
references, 
legends, 
etc.). 


5.1 
Contents 
of 
first 
page 
of 
manuscript 
The 
first 
page 
of 
the 
manuscript 
should 
contain 
only 
the 
following: 


1) 
Title 
of 
the 
paper 
containing 
only 
the 
keywords 
pertaining 
to 
the 
subject 
matter. 
Standard 
abbreviations 
may 
be 
used 
in 
the 
title. 


2) 
Full 
names 
(including 
first 
name) 
of 
the 
authors 
and 
the 
name 
of 
the 
institute. 
If 
the 
publication 
originates 
from 
several 
institutes 
the 
affiliations 
of 
all 
authors 
should 
be 
clearly 
stated 
by 
using 
superscript 
numbers 
after 
the 
name 
and 
before 
the 
institute. 


3) 
Name 
(and 
title) 
and 
full 
postal 
address 
of 
the 
author 
to 
whom 
all 
correspondence 
(including 
galley 
proofs) 
is 
to 
be 
sent. 
E-mail 
address 
and 
fax 
number 
must 
be 
included 
to 
speed 
up 
communication. 


4) 
A 
list 
of 
abbreviations 
used 
in 
the 
paper 
excluding 
standard 
abbreviations 
(see 
list 
of 
“Standard 
Abbreviations”, 
Section 
8). 


5) 
Keywords 
(max. 
5, 
in 
alphabetical 
order). 


5.2 
Abstract 
The 
second 
and 
(if 
necessary) 
third 
page 
of 
the 
manuscript 
should 
contain 
the 
abstract 
only. 
This 
must 
be 
self-explanatory 
and 
intelligible 
without 
reference 
to 
the 
text. 
It 
should 
not 
exceed 
200 
words 
for 
research 
and 
review 
articles. 
Abstracts 
for 
rapid 
communications, 
technical 
and 
dataset 
briefs, 
viewpoint 
articles, 
and 
reports 
should 
not 


ª 
2008 
WILEY-VCH 
Verlag 
GmbH 
& 
Co. 
KGaA, 
Weinheim 


exceed 
80 
words. 
Abbreviations, 
but 
not 
standard 
abbreviations, 
must 
be 
written 
in 
full 
when 
first 
used. 
Any 
references 
cited 
must 
be 
given 
in 
full. 


5.3 
Division 
into 
sections 
Manuscripts 
should 
be 
divided 
into 
the 
following 
sections: 


“1 
Introduction”: 
containing 
a 
description 
of 
the 
problem 
under 
investigation 
and 
a 
brief 
survey 
of 
the 
existing 
literature 
on 
the 
subject. 


“2 
Materials 
and 
methods”: 
for 
special 
materials 
and 
equipment, 
the 


manufacturer’s 
name 
and, 
if 
possible, 
the 
location 
should 
be 
pro


vided. 


“3 
Results” 


“4 
Discussion” 


“5 
References” 


Sections 
3 
and 
4 
may 
be 
combined 
and 
should 
then 
be 
followed 
by 
a 
short 
section 
entitled 
“Concluding 
remarks”. 
Subdivisions 
of 
sections 
should 
be 
indicated 
by 
numbered 
subheadings. 


5.4 
References 
References 
should 
be 
numbered 
sequentially 
in 
the 
order 
in 
which 
they 
are 
cited 
in 
the 
text. 
The 
numbers 
should 
be 
set 
in 
brackets, 
thus 
[2, 
18]. 
References 
are 
to 
be 
collected 
in 
numerical 
order 
at 
the 
end 
of 
the 
manuscript 
under 
the 
heading 
“References”; 
they 
should 
also 
be 
typed 
with 
double 
spacing 
throughout. 
Papers 
with 
multiple 
authors 
should 
be 
limited 
to 
listing 
five 
authors. 
Where 
there 
are 
more 
than 
five 
authors, 
the 
first 
four 
should 
be 
listed, 
followed 
by 
et 
al. 
Please 
include 
the 
title 
of 
the 
manuscript 
in 
full 
followed 
by 
a 
full 
stop. 
Journal 
names 
should 
be 
abbreviated 
according 
to 
the 
practice 
of 
PubMed. 
The 
abbreviated 
title 
and 
the 
volume 
number 
should 
be 
in 
italics. 
Please 
note 
the 
following 
examples. 


Journals: 


[1] 
Hu, 
J., 
Qian, 
J., 
Borisov, 
O., 
Pan, 
S. 
et 
al., 
Optimized 
proteomic 
analysis 
of 
a 
mouse 
model 
of 
cerebellar 
dysfunction 
using 
amine-
specific 
isobaric 
tags. 
Proteomics 
2006, 
6, 
4321–4334. 
[2] 
Vosseller, 
K., 
Proteomics 
of 
Alzheimer’s 
disease: 
Unveiling 
protein 
dysregulation 
in 
complex 
neuronal 
systems. 
Proteomics 
Clin. 
Appl. 
2007, 
1, 
1351–1361. 
Other 
serial 
publications 
such 
as 
“Advances 
in 
Protein 
Chemistry” 
should 
be 
cited 
in 
the 
same 
manner 
as 
journals. 


Books: 


[3] 
Elves, 
M. 
W., 
The 
Lymphocytes, 
Lloyd-Luke 
Ltd., 
London 
1972. 
Chapter 
in 
a 
book: 


[4] 
Mller, 
E., 
Greaves, 
M. 
F., 
in: 
Mkel, 
O., 
Cross, 
A., 
Kosunen, 
T. 
U. 
(Eds.), 
Cell 
Interactions 
and 
Receptor 
Antibodies 
in 
Immune 
Responses, 
Academic 
Press, 
New 
York 
1971, 
pp. 
101–125. 
Allusions 
to 
“unpublished 
observations”, 
papers 
“to 
be 
published” 
or 
“submitted 
for 
publication” 
and 
the 
like 
should 
be 
part 
of 
the 
text, 
in 
parentheses. 
Material 
“in 
press” 
should 
be 
entered 
under 
references 
along 
with 
the 
DOI 
(Digital 
Object 
Identifier), 
if 
available. 
Posters 
and 
abstracts 
in 
meetings 
books 
must 
not 
be 
cited 
unless 
they 
are 
generally 
accessible. 
Responsibility 
for 
the 
accuracy 
of 
bibliographic 
references 
rests 
entirely 
with 
the 
author. 


Please 
not 
that 
website 
addresses 
must 
not 
be 
included 
as 
a 
reference, 
but 
should 
be 
inserted 
in 
the 
text 
directly 
after 
the 
data 
to 
which 
they 
refer. 


A 
link 
to 
the 
latest 
End 
Note 
style 
sheet 
can 
be 
found 
on 
the 
homepage 
www.proteomics-journal.com 
under 
the 
link 
For 
Authors. 


5.5 
Acknowledgements 
Acknowledgements 
as 
well 
as 
information 
regarding 
funding 
sources 
should 
be 
provided 
on 
a 
separate 
page 
and 
will 
appear 
at 
the 
end 
of 
the 
text 
(before 
the 
“References”). 


www.proteomics-journal.com 



5.6 
Conflict 
of 
interest 
statement 
All 
authors 
must 
declare 
financial/commercial 
conflicts 
of 
interest. 
Even 
if 
there 
are 
none, 
this 
should 
be 
stated 
in 
a 
separate 
paragraph 
following 
on 
from 
the 
acknowledgements 
section. 
This 
is 
a 
mandatory 
requirement 
for 
all 
articles. 


5.7 
Tables 
Tables 
with 
suitable 
captions 
at 
the 
top 
and 
numbered 
with 
Arabic 
numerals 
should 
be 
collected 
at 
the 
end 
of 
the 
text 
on 
separate 
sheets 
(one 
page 
per 
table). 
Column 
headings 
should 
be 
kept 
as 
brief 
as 
possible 
and 
indicate 
units. 
Footnotes 
to 
tables 
should 
be 
indicated 
with 
a), 
b), 
c) 
etc. 
and 
typed 
on 
the 
same 
page 
as 
the 
table. 


5.8 
Supporting 
information 
Extensive 
tables 
should 
be 
published 
online 
as 
supporting 
information. 
This 
material 
will 
not 
be 
typeset 
so 
authors 
should 
prepare 
this 
in 
the 
final 
form. 
Also 
for 
this 
reason 
there 
will 
be 
no 
galley 
proofs 
of 
this 
material. 
Supporting 
information 
will 
be 
made 
freely 
available 
on 
the 
web 
(similar 
to 
the 
table 
of 
contents 
and 
the 
article 
abstracts). 
Authors 
are 
permitted 
to 
place 
this 
material 
on 
their 
homepages 
when 
they 
are 
setting 
up 
a 
link 
to 
the 
full-text 
version 
of 
the 
article 
in 
Wiley 
InterScience. 


Further, 
other 
files 
may 
be 
submitted 
as 
supporting 
information 
(e.g. 
animations, 
video 
sequences). 
All 
supporting 
information 
will 
also 
undergo 
the 
peer-review 
process. 
Thus, 
this 
material 
has 
to 
be 
submitted 
electronically 
along 
with 
the 
main 
body 
of 
the 
article. 
It 
is 
in 
the 
hands 
of 
the 
Editor-in-Chief 
to 
decide 
which 
part 
of 
the 
manuscript 
will 
be 
published 
as 
supporting 
information. 


Protein 
identification 
results, 
expression 
data, 
and 
mass 
spectrometry 
peak 
lists 
should 
also 
be 
submitted 
as 
supporting 
information, 
and 
may 
be 
identical 
to 
data 
deposited 
in 
a 
public 
database. 
Note 
that 
all 
data 
must 
be 
in 
processed, 
not 
raw, 
form. 
Data 
should 
be 
deposited 
in 
public, 
open 
access 
databases, 
formatted 
according 
to 
conventions 
of 
the 
relevant 
communities 
prior 
to 
manuscript 
submission, 
and 
database 
accession 
numbers 
provided 
in 
the 
manuscript. 
In 
particular, 
novel 
protein 
sequences 
should 
be 
deposited 
in 
UniProt 
(www.uniprot. 
org); 
molecular 
interactions 
in 
an 
IMEx 
partner 
database 
(imex.sf. 
net); 
and 
protein 
identification 
data 
in 
PRIDE 
(www.ebi.ac.uk/pride), 
World-2DPAGE 
(www.expasy.org/world-2dpage/), 
or 
a 
comparable 
database. 


5.9 
Figures 
and 
legends 
Diagrams 
and 
photographs 
should 
be 
submitted 
as 
separate 
files. 
Upon 
acceptance 
of 
the 
paper 
hardcopies 
of 
fine 
quality 
suitable 
for 
reproduction 
should 
be 
sent 
to 
the 
Editorial 
Office 
(address 
see 
Section 
2). 
Figures 
should 
be 
numbered 
consecutively 
with 
Arabic 
numerals 
in 
the 
order 
of 
their 
appearance. 
Figures 
should 
be 
submitted 
in 
a 
format 
which 
can 
be 
reduced 
to 
a 
width 
of 
50–80 
mm 
or 
120–170 
mm, 
and 
symbols 
and 
labels 
to 
a 
height 
of 
2.0 
mm 
(after 
reduction). 
In 
electropherograms 
presented 
horizontally, 
the 
anode 
should 
be 
on 
the 
left 
while 
in 
vertical 
presentations 
the 
anode 
should 
be 
at 
the 
bottom. 
Two-dimensional 
presentations, 
e.g. 
with 
isoelectric 
focusing 
and 
sodium 
dodecyl 
sulfate-electrophoresis 
in 
two 
dimensions, 
are 
thus 
presented 
consistently 
with 
the 
standard 
coordinate 
system. 
Each 
figure 
is 
to 
be 
accompanied 
by 
a 
legend 
which 
should 
be 
self-explanatory. 
The 
legends 
should 
not 
appear 
under 
the 
figures 
but 
be 
collected 
and 
typewritten 
with 
double 
spacing 
following 
the 
references. 


Color 
figures 
can 
be 
reproduced: 
however 
authors 
will 
be 
charged 
for 
additional 
costs 
incurred 
for 
the 
reproduction 
of 
color 
(see 
Section 
2). 


5.10 
Image 
manipulation 
Manipulation 
of 
images 
is 
strongly 
discouraged 
and 
all 
figures 
must 
accurately 
reflect 
the 
original 
data. 
Information 
should 
not 
be 
enhanced, 
eliminated, 
added, 
obscured 
or 
moved. 
In 
cases 
where 
manipulation 
is 
unavoidable, 
this 
should 
be 
clearly 
detailed 
in 
the 
Figure 
legend. 
All 
instruments, 
software 
and 
processes 
used 
to 
obtain 
the 
images 
must 
be 
fully 
detailed 
in 
the 
manuscript 
either 
in 
the 
Figure 
legends 
or 
the 
Materials 
and 
Methods. 
Acceptable 
image 
manipulation 


ª 
2008 
WILEY-VCH 
Verlag 
GmbH 
& 
Co. 
KGaA, 
Weinheim 


includes 
uniformly 
adjusting 
the 
contrast 
of 
an 
entire 
image, 
and 
any 
control 
images, 
ensuring 
that 
all 
original 
data, 
including 
the 
background, 
remains 
visible 
and 
that 
no 
new 
features 
are 
introduced. 
Cropping 
of 
gels, 
or 
re-positioning 
of 
lanes/fields, 
is 
permitted 
providing 
that 
all 
alterations 
are 
clearly 
indicated 
by 
the 
use 
of 
dividing 
lines 
in 
the 
image 
itself, 
vital 
data 
are 
not 
removed 
and 
an 
explanation 
of 
the 
alterations 
is 
included 
in 
the 
Figure 
legend. 
Unacceptable 
manipulation 
includes, 
but 
is 
not 
limited 
to, 
the 
enhancement 
of 
one 
feature/ 
band 
over 
others, 
removal 
of 
background 
noise/bands 
etc. 
Authors 
must 
be 
able 
to 
produce 
all 
data 
in 
their 
raw 
format 
upon 
editorial 
request. 


5.11 
Biographic 
material 
Corresponding 
authors 
of 
review 
and 
viewpoint 
articles 
are 
invited 
to 
submit 
a 
portrait 
photograph 
of 
themselves 
and 
a 
short 
biographical 
text 
(no 
more 
than 
80 
words) 
which 
will 
appear 
at 
the 
very 
end 
of 
the 
article. 


5.12 
Structural 
formulae 
Structural 
formulae 
should 
be 
drawn 
in 
the 
manuscript 
in 
the 
position 
where 
they 
belong. 
They 
must 
be 
numbered 
in 
consecutive 
order 
with 
the 
other 
figures. 


5.13 
Equations 
Mathematical 
and 
chemical 
equations 
are 
to 
be 
written 
in 
the 
manuscript 
at 
the 
place 
in 
which 
they 
belong 
and 
should 
be 
marked 
by 
Arabic 
numerals 
in 
parentheses 
in 
the 
right 
margin 
in 
the 
order 
of 
their 
appearance. 


5.14 
Abbreviations 
Abbreviations 
are 
hindrances 
to 
a 
reader 
working 
in 
a 
field 
other 
than 
that 
of 
the 
author, 
and 
to 
abstractors. 
Therefore, 
their 
use 
should 
be 
restricted 
to 
a 
minimum. 
Abbreviations 
should 
be 
introduced 
only 
when 
repeatedly 
used. 
Abbreviations 
used 
only 
in 
a 
table 
or 
a 
figure 
may 
be 
defined 
in 
the 
legend. 
Standard 
abbreviations 
may 
be 
used 
in 
the 
title 
and 
keywords. 
If 
nonstandard 
abbreviations 
are 
used 
in 
the 
Abstract 
they 
should 
be 
defined 
in 
the 
Abstract, 
in 
the 
list 
of 
abbreviations 
of 
the 
manuscript, 
as 
well 
as 
when 
first 
used 
in 
the 
body 
of 
the 
paper. 


Section 
8 
at 
the 
end 
of 
these 
instructions 
contains 
the 
list 
of 
standard 
abbreviations 
which 
may 
be 
used 
without 
definition 
in 
the 
articles 
published 
in 
PROTEOMICS. 


5.15 
Ethics 
If 
the 
manuscript 
describes 
experiments 
using 
animals, 
the 
permission 
of 
the 
national 
or 
local 
authorities 
(giving 
the 
permission 
or 
the 
accreditation 
number 
of 
the 
laboratory 
and 
of 
the 
investigator) 
should 
be 
stated. 
If 
no 
such 
rules 
or 
permission 
are 
stipulated 
in 
the 
particular 
country, 
this 
must 
also 
be 
mentioned 
in 
the 
paper. 
In 
the 
case 
of 
human 
studies, 
it 
should 
be 
stated 
that 
local 
ethical 
committee 
approval 
has 
been 
received 
and 
that 
the 
informed 
consent 
of 
all 
participating 
subjects 
was 
obtained. 
These 
statements 
must 
be 
confirmed 
in 
the 
cover 
letter. 


5.16 
Sharing 
of 
materials 
All 
materials 
and 
reagents 
that 
are 
not 
commercially 
available 
(antibodies, 
cell 
lines, 
constructs 
etc.) 
and 
associated 
protocols 
detailed 
in 
manuscripts 
published 
in 
PROTEOMICS 
are 
to 
be 
freely 
available 
to 
academic 
researchers 
in 
a 
timely 
manner 
upon 
request. 
The 
authors 
agree 
to 
this 
condition 
by 
submitting 
a 
manuscript 
to 
PROTEOMICS. 


6 
Proofs 
and 
reprints 


Before 
publication 
authors 
will 
receive 
page 
proofs 
via 
e-mail 
in 
PDF 
low 
resolution 
file 
format, 
together 
with 
instructions 
and 
a 
reprint 
order 
form, 
also 
as 
PDF 
files. 
The 
page 
proofs 
and 
the 
reprint 
order 
form 
should 
be 
printed 
out. 
The 
proofs 
should 
be 
carefully 
corrected 
following 
the 
instructions. 
In 
particular, 
authors 
should 
answer 
any 


www.proteomics-journal.com 



editing 
queries. 
The 
reprint 
order 
form 
should 
be 
filled 
out 
(even 
if 
additional 
reprints 
are 
not 
required), 
and 
both 
should 
be 
returned, 
preferably 
by 
fax, 
to 
the 
address 
given 
in 
Section 
2. 


Authors 
will 
be 
charged 
for 
extensive 
alterations 
of 
their 
article. 
Reprints 
can 
be 
ordered 
at 
prices 
shown 
on 
the 
reprint 
order 
form. 
Upon 
publication 
the 
submitting 
author 
(listed 
under 
“Correspondence”) 
will 
receive 
a 
complimentary 
copy 
of 
the 
issue 
containing 
the 
article. 


7 
Funding 
information 


7.1 
NIH 
Authors 
On 
behalf 
of 
our 
authors 
who 
are 
also 
NIH 
grantees, 
Wiley 
will 
deposit 
in 
PMC 
at 
the 
same 
time 
that 
the 
article 
is 
published 
in 
our 
journal 
the 
peer-reviewed 
version 
of 
the 
author’s 
manuscript. 
Wiley 
will 
stipulate 
that 
the 
manuscript 
may 
be 
available 
for 
“public 
access” 
in 
PMC 
12 
months 
after 
the 
date 
of 
publication. 


By 
assuming 
this 
responsibility, 
Wiley 
will 
ensure 
that 
authors 
are 
in 
compliance 
with 
the 
NIH 
request, 
as 
well 
as 
make 
certain 
the 
appropriate 
version 
of 
the 
manuscript 
is 
deposited. 


When 
an 
NIH 
grant 
is 
mentioned 
in 
the 
Acknowledgments, 
Wiley 
will 
assume 
that 
the 
author 
wants 
the 
manuscript 
deposited 
into 
PMC, 
unless 
the 
author 
states 
otherwise. 
The 
author 
can 
communicate 
this 
via 
email, 
or 
a 
note 
in 
the 
manuscript. 
The 
version 
of 
the 
manuscript 
that 
Wiley 
sends 
to 
PMC 
will 
be 
the 
accepted 
version. 


Because 
Wiley 
is 
taking 
the 
responsibility 
for 
sending 
the 
manuscripts 
to 
PMC, 
in 
order 
to 
ensure 
an 
orderly 
process, 
authors 
should 
not 
deposit 
Wiley 
articles 
to 
PMC 
themselves. 
Authors 
should 
not 
make 
corrections 
to 
their 
Wiley-deposited 
manuscripts 
in 
PMC. 


Wiley 
reserves 
the 
right 
to 
change 
or 
rescind 
this 
policy. 


For 
further 
information, 
please 
see 
the 
NIH 
Policy 
on 
Public 
Access, 
located 
at 
http://publicaccess.nih.gov/ 


7.2 
Funded 
access 
Wiley 
is 
now 
offering 
a 
funded 
access 
service 
for 
authors 
of 
journal 
articles 
whose 
funding 
agency 
requires 
deposit 
of 
an 
article 
in 
an 
archive. 
Interested 
authors 
should 
go 
to 
http://www3.interscience. 
wiley.com/authorresources/funded_access.html 
for 
further 
information. 


ª 
2008 
WILEY-VCH 
Verlag 
GmbH 
& 
Co. 
KGaA, 
Weinheim 
www.proteomics-journal.com 



8 
Standard 
abbreviations 


The 
abbreviations 
as 
listed 
below 
may 
be 
used 
without 
definition 
in 
the 
articles 
published 
in 
PROTEOMICS. 


NEPHGE 
nonequilibrium 
pH 
gradient 


electrophoresis 
NMR 
nuclear 
magnetic 
resonance 
NP-40 
Nonidet 
P-40 
od 
outside 
diameter 


OD 
optical 
density 
OFAGE 
orthogonal 
field 
alternation 
gel 


electrophoresis 
ORF 
open 
reading 
frame 
PAGE 
polyacrylamide 
gel 
electrophoresis 
PBS 
phosphate-buffered 
saline 
PCA 
principal 
components 
analysis 
PCR 
polymerase 
chain 
reaction 
PDMS 
polydimethylsiloxane 
PED 
pulsed 
electrochemical 
detection 
PEG 
polyethylene 
glycol 
PFGE 
pulsed-field 
gel 
electrophoresis 
PFU 
plaque-forming 
units 
pI 
isoelectric 
point 
PMF 
peptide 
mass 
fingerprinting 
PMS 
phenazine 
methosulfate 
PMSF 
phenylmethylsulfonyl 
fluoride 
PMT 
photomultiplier 
tube 
PSD 
post-source 
decay 
PTFE 
polytetrafluoroethylene 
PTH 
phenylthiohydantoin 
PTM 
post-translational 
modification 
PVA 
polyvinyl 
alcohol 
PVDF 
polyvinylidene 
difluoride 
PVP 
polyvinylpyrrolidone 
RFLP 
restriction 
fragment 
length 


polymorphism 
RIA 
radioimmunoassay 
ROS 
reactive 
oxygen 
species 
RP 
reversed 
phase 
rpm 
revolutions 
per 
minute 
RSD 
relative 
standard 
deviation 
RT-PCR 
reverse 
transcriptase-PCR 
SAGE 
serial 
analysis 
of 
gene 
expression 
SD 
standard 
deviation 
SDS 
sodium 
dodecyl 
sulfate 
SEC 
size-exclusion 
chromatography 
SELDI 
surface-enhanced 
laser 
desorption/ 


ionization 
SEM 
standard 
error 
of 
the 
mean 
SIM 
selected 
ion 
monitoring 
S/N 
signal-to-noise 
ratio 
SPE 
solid-phase 
extraction 
SPR 
surface 
plasmon 
resonance 
SSCP 
single-strand 
conformation 


polymorphism 
ssDNA 
single-stranded 
DNA 
SSP 
sample 
spot 
number 
STR 
short 
tandem 
repeat 
%T 
total 
gel 
concentration 
(acrylamide 


plus 
cross-linking 
agent; 
g/100 
mL) 
TBS 
Tris-buffered 
saline 
TCA 
trichloroacetic 
acid 
TEMED 
N,N,N’,N’-tetramethylethylenedia


mine 
TFA 
trifluoroacetic 
acid 
THF 
tetrahydrofuran 
TIC 
total 
ion 
current 
TLC 
thin-layer 
chromatography 
TOF 
time 
of 
flight 
Tris 
tris(hydroxymethyl)aminomethane 
URL 
uniform 
resource 
locator 
Vh 
volt6hours 


A 
ACES 


ACN 
A/D 
amu 
ANOVA 
API 
AUC 
BCIP 
Bis 
bp 
BSA 
%C 
CAPS 


CBB 
CCD 
CE 
CEC 
CFE 
CHAPS 


CHCA 
CHES 


CID 
CIEF 
CMC 
Con 
A 
CNS 
cpm 
CTAB 
CV 
CZE 
1-D 
2-D 
Da 
2-DE 
DIGE 


DGGE 


DMEM 
DMF 
DMSO 
DOC 
dsDNA 
DTT 
ECL 
EDTA 
EEO 
EGTA 


EKC 
ELISA 


EOF 
ER 
ESI 
EST 
FAB 
FBS 


absorbance 
2-[(2-amino-2-oxoethyl)amino] 
ethanesulfonic 
acid 
acetonitrile 
analog 
to 
digital 
converter 
atomic 
mass 
unit 
analysis 
of 
variance 
atmospheric 
pressure 
ionization 
area 
under 
curve 
5-bromo-4-chloro-3-indolyl 
phosphate 
N,N’-methylenebisacrylamide 
base 
pairs 
bovine 
serum 
albumin 
cross-linking 
agent 
(g/100 
mL)/%T 
3-(cyclohexylamino)


1-propanesulfonic 
acid 
Coomassie 
Brilliant 
Blue 
charge-coupled 
device 
capillary 
electrophoresis 
capillary 
electrochromatography 
continuous 
flow 
electrophoresis 
3-[(3-cholamidopropyl)dimethyl


amonio]-1-propanesulfonate 
a-cyano-4-hydroxycinnamic 
acid 
2-(N-cyclohexylamino)ethane


sulfonic 
acid 
collision-induced 
dissociation 
capillary 
isoelectric 
focusing 
critical 
micelle 
concentration 
Concanavalin 
A 
central 
nervous 
system 
counts 
per 
minute 
cetyltrimethylammonium 
bromide 
coefficient 
of 
variation 
capillary 
zone 
electrophoresis 
one-dimensional 
two-dimensional 
dalton 
(molecular 
mass) 
two-dimensional 
gel 
electrophoresis 
fluorescence 
difference 
gel 
electro


phoresis 


denaturing 
gradient 
gel 
electrophoresis 
Dulbecco’s 
modified 
Eagle 
medium 
N,N-dimethylformamide 
dimethyl 
sulfoxide 
sodium 
deoxycholate 
double-stranded 
DNA 
dithiothreitol 
enhanced 
chemiluminescence 
ethylenediaminetetraacetic 
acid 
electroendosmosis 
ethylene 
glycol-bis(ß-aminoethyl


ether)-N,N,N’,N’-tetraacetic 
acid 
electrokinetic 
chromatography 
enzyme-linked 
immunosorbent 


assay 
electroosmotic 
flow 
endoplasmic 
reticulum 
electrospray 
ionization 
expressed 
sequence 
tag 
fast 
atomic 
bombardment 
fetal 
bovine 
serum 


FCS 
FIGE 
FITC 
FT-ICR 


GC 
GIF 
GRAVY 
GSH 
GST 
HE 
HEPES 


HPCE 


HPLC 


HRP 
HSA 
HSP 
HTML 
HUPO 
HVR 
ICAT 
ICR 
id 
IEF 
Ig 
IMAC 
IPG 
IPTG 
IT 
iTRAQ 


kbp 
kDa 
LC 
LED 
LOD 
LOQ 
mAb 
MALDIMS 


Mbp 
MEKC 


MES 


MHC 
MOPS 


Mr 


MS 
MS/MS 


m/z 


NBT 
NC 


fetal 
calf 
serum 
field 
inversion 
gel 
electrophoresis 
fluorescein 
isothiocyanate 
Fourier 
transform-ion 
cyclotron 


resonance 
gas 
chromatography 
graphic 
interchange 
format 
grand 
average 
of 
hydrophobicity 
glutathione 
glutathione-S-transferase 
hematoxylin 
and 
eosin 
N-(2-hydroxyethyl)piperazine-2’(
2-ethanesulfonic 
acid) 
high-performance 
capillary 


electrophoresis 


high-performance 
liquid 
chromatography 
horseradish 
peroxidase 
human 
serum 
albumin 
heat 
shock 
protein 
hypertext 
mark-up 
language 
Human 
Proteome 
Organisation 
hypervariable 
region 
isotope-coded 
affinity 
tag 
ion 
cyclotron 
resonance 
inside 
diameter 
isoelectric 
focusing 
immunoglobulin 
immobilized 
metal 
affinity 
capture 
immobilized 
pH 
gradient 
isopropyl-b-D-thiogalactopyranoside 
ion 
trap 
isobaric 
tag 
for 
relative 
and 
absolute 
quantitation 
kilobase 
pairs 
kilodalton 
(molecular 
mass) 
liquid 
chromatography 
light-emitting 
diode 
limit 
of 
detection 
limit 
of 
quantitation 
monoclonal 
antibody 


matrix-assisted 
laser 
desorption/ 
ionization–mass 
spectrometry 
megabase 
pairs 
micellar 
electrokinetic 
capillary 
chromatography 
2-(N-morpholino)ethanesulfonic 
acid 
major 
histocompatibility 
complex 
3-(N-morpholino)propanesulfonic 
acid 
relative 
molecular 
mass 
(dimensionless) 


mass 
spectrometry 
tandem 
mass 
spectrometry 
mass-to-charge 
ratio 
nitroblue 
tetrazolium 
nitrocellulose 


ª 
2008 
WILEY-VCH 
Verlag 
GmbH 
& 
Co. 
KGaA, 
Weinheim 
www.proteomics-journal.com 



